图书简介
In the last decade, great advances have been made in fundamental research and in the applications of bioluminescence and chemiluminescence. These techniques have become vital tools for laboratory analysis. Bioluminescence imaging has emerged as a powerful new optical imaging technique, offering real-time monitoring of spatial and temporal progression of biological processes in living animals. Bioluminescence resonance energy transfer (BRET) methodology has also emerged as a powerful technique for the study of protein–protein interactions. Luciferase reporter gene technology facilitates monitoring of gene expression and is used to probe molecular mechanisms in the regulation of gene expression. Chemiluminescence detection and analysis have also found diverse applications in life science research; for example, chemiluminescent labels and substrates are now widely used in immunoassay and nucleic acid probe-based assays.
The latest advances in this exciting field, from fundamental research to cutting-edge applications, are explored in this most recent volume of the biannual symposium series, the Proceedings of the 15th International Symposium on Bioluminescence and Chemiluminescence. The volume highlights advances in fundamental knowledge of luciferase-based bioluminescence, photoprotein-based bioluminescence, fundamental aspects and applications of chemiluminescence, luminescence imaging, fluorescence quantum dots and other inorganic fluorescent materials, phosphorescence and ultraweak luminescence, and instrumentation for measurement and imaging of luminescence.
Key Features
• Contains detailed coverage of the latest developments in bioluminescence and chemiluminescence research
• Provides important information on recent applications and instrumentation developed for bioluminescence and chemiluminescence
• Describes experimental procedures and protocols in bioluminescence and chemiluminescence
• Covers phosphorescent and fluorescent materials
Progress, Perspectives and Problems in Basic Aspects of Bioluminescence (J W Hastings); Bioluminescence of Sharks, A Case Study: Etmopterus spinax (J M Claes & J Mallefet); Chemiexcitation Mechanism for Cypridina (Vargula) and Aequorea Bioluminescence (T Hirano et al.); Study on ATP-Dependent Luminescence Reaction of the Arm Light Organs of the Luminous Squid Watasenia scintillans (K Teranishi & O Shimomura); Bioluminescent Assay of Antibiotic Susceptibility of Clinical Samples (V G Frundzhyan & N N Ugarova); BART: Smart Biochemistry, Bright Bioluminescence, Low-Cost Hardware (O A Gandelman et al.); Mass Spectrometric Approach to Elucidation of Chemiexcitation of Dioxetanes (H K Ijuin et al.); Analytical Challenges for Luminescence-based Point-of-Care Testing Devices in Biomedical Diagnostics (A Roda et al.); Molecular Imprinted Polymer-based Chemiluminescence Sensors (Z Zhang); Conjugates of (Acridinium)x-BSA-Anti-精装V Core to Enhance the Detection of 精装V Core Antigen (C D Chang et al.); Simultaneous Multiplex Bio- and Chemiluminescent Enzyme Immunoassay for PCR Products Derived from Genetically Modified Papaya (K Ito et al.); A Sensitive Micellar-Enhanced Chemiluminescence Method for the Determination of Ofloxacin by Flow Injection Analysis (H Ma et al.); Development and Optimization of a Fast and Sensitive ELISA for Polio D-Antigen Using a GZ11-based Signal Reagent (G Zomer & M Hamzink); Detection of Xanthomonas oryzae pv. Oryzicola by Electrochemiluminescence Polymerase Chain Reaction Method (J Wei & L Zhang); A Novel Multicolor Fluorescent Protein from the Soft Coral Scleronephthya gracillima Kuekenthal (Y Kato et al.); Identification of Developmental Enhancers Using Targeted Regional Electroporation (TREP) of Evolutionarily Conserved Regions (C U Pira et al.); Quantum Dots as Fluorescent Resonance Energy Transfer Donors in Antibody-Antigen Systems (S Hu et al.); Synthesis and Photoluminescence of Green-Emitting X2-(Y,Gd)2SiO5:Tb3+ Phosphor Under VUV Excitation (Z H Zhang et al.); The Measurement of Cytosolic ATP During Apoptosis: Bioluminescence Imaging at the Single-Cell Level (R Akiyoshi & H Suzuki); Development of a New Device for Ultrasensitive Electrochemiluminescence Microscope Imaging (L S Dolci et al.); Delayed Fluorescence and Optical Molecule Imaging Techniques for Detecting the Stress Response of Plants to High Temperature (L Zhang & F Wen); Microemulsion Sensitized Determination of BSA with 3-(4’-methylphenyl)-5-(2’-sulfophenylazo) Rhodanine by Resonance Rayleigh Scattering Method (S Ge et al.); The Mechanism of Photon Emission of Bio-Tissues and Its Properties (X-F Pang & X-Y Cao); and other papers.
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