图书简介

Rapid and Highly Efficient Isolation and Purification of Human Induced Pluripotent Stem Cells.-Artificial Activation of Murine Oocytes using Strontium to Derive Haploid and Diploid Parthenotes.-Generation of Human iPSC from Small Volume Peripheral Blood Samples.-Distinguishing between Endodermal and Pluripotent Stem Cell Lines during Somatic Cell Reprogramming.-Measuring Early Germ-Layer Specification Bias in Human Pluripotent Stem Cells.-Detection of Soluble and Insoluble Protein Species in Patient-Derived iPSCs.-Extracellular Flux Analysis of Mitochondrial Function in Pluripotent Stem Cells.-Assessment of Endothelial-to-Hematopoietic Transition of Individual Hemogenic Endothelium and Bulk Populations in Defined Conditions.-Homogenous Differentiation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells.-Differentiation of Human Induced Pluripotent Stem Cells into Cortical Neurons to Advance Precision Medicine.-Differentiation of iPS-Cells into Peripheral Sensory Neurons.-Culture of Human iPSC-Derived Motoneurons in Compartmentalized Microfluidic Devices and Quantitative Assays for Studying Axonal Phenotypes.-iPS Cell-Differentiation into Brain Microvascular Endothelial Cells.-Chromatin Immunoprecipitation in Human Pluripotent Stem Cell-Derived 3D Organoids to Analyze DNA-Protein Interactions.-Generation of Embryonic Origin-Specific Vascular Smooth Muscle Cells from Human Induced Pluripotent Stem Cells.-Generation of Salivary Gland Organoids from Mouse Embryonic Stem Cells.-In Vitro Generation of Heart Field Specific Cardiomyocytes.-Isolation and Characterization of Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells.-Identification and Validation of CRISPR/Cas9 Off-Target Activity in Hematopoietic Stem and Progenitor Cells.-Genome Engineering of Hematopoietic Stem Cells using CRISPR/Cas9 System.-Generation of Rat Neural Stem Cells to Produce Different Astrocyte Phenotypes.-In Situ Quantification and Isolation of Müller Glial Cells by Fluorescence Activated Cell Sorting from the Regenerating Larval Zebrafish Retina.-Quantification and Cloncal Culture of Neural Stem Cells from the Hippocampus of Adult Mouse.-Reprogramming Mouse Oviduct Epithelial Cells using In Vivo Electroporation and CRISPR/Cas9 Mediated Genetic Manipulation.-Generation of Human Liver Chimeric Mice and Harvesting of Human Hepatocytes from Mouse Livers.-Application of 3D Culture Assays to Study Breast Morphogenesis, Epithelial Plasticity, and Cellular Interactions in an Epithelial Progenitor Cell Line.-A Unified Protocol to Streamline Molecular and Cellular Analysis for Three-Dimensional Cell Cultures.-Mesenchymal Stem Cell Seeding on 3D Scaffolds.-Assaying Candidate Human Skin Keratinocyte Stem Cells by Determining their Long-Term Serial Proliferative Output in Culture.-Protocol for the Detection of Organoid-Initiating Cell Activity in Patience-Derived Single Fallopian Tube Epithelial Cells.-Quantification of Mucle Stem Cell Differentiation using Live-Cell Imaging and Eccentricity Measures.-The Enrichment of Breast Cancer Stem Cells from MCF7 Breast Cancer Cell Line using Spheroid Culture Technique.-Assessment of Breast Cancer Stem Cell Activity using a Spheroid Formation Assay.-Enrichment of Cancer Stem Cells in a Tumor Sphere Assay.-In Vitro Quantification of Cancer Stem Cells using a Mammosphere Formation Assay.-Designing Genetically Engineered Mouse Models (GEMMSs) using CRISPS Mediated Genome Editing.-Assays for the Spectrum of Circulating Tumor Cells.-Limiting Dilution Tumor Initiation Assay: An In Vitro Approach for the Study of Cancer Stem Cells.-Orthotopic Xenografts of Colorectal Cancer Stem Cells.

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